Journal: Brain research bulletin

Article Title: Effects of Interleukin-19 overexpression in the medial prefrontal cortex on anxiety-related behaviors, BDNF expression and p38/JNK/ERK pathways.

doi: 10.1016/j.brainresbull.2024.110952

Figure Lengend Snippet: Fig. 7. Western blot images and quantitative data showing protein and phosphorylation alterations of p38, JNK, and Erk in the mPFC of AAV-CaMKII-GFP (NC) and AAV-CaMKII-IL-19-GFP (IL-19) mice. (A) Representative western blot images. Quantitative data showing (B) protein levels and (C) phosphorylated protein levels of p38, JNK, and Erk. n = 5 per group. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group.

Article Snippet: The membrane was blocked at room temperature for 2 h using 5 % bovine serum albumin (BSA) in 1×TBST buffer (150 mM NaCl, 20 mM Tris-HCl [pH 7.4], and 0.1 % Tween-20) and incubated at 4 ◦C overnight with the following primary antibodies diluted in prepared blocking buffer: anti-IL-19 (1:500, ab154187, Abcam, Cambridge, UK), anti-BDNF (1:1000, ab108319, Abcam), anti-PSD95 (1:500, sc-32290, Santa Cruz Biotechnology, CA, USA), anti-IL-20Rα (1:500, bs-2619R, Bioss antibody, Woburn, MA, USA), anti-phospho-p38 MAPK(1:1000, #9215, Cell Signaling Technology, Danvers, MA, USA), anti-p38 MAPK (1:1000, #9212, Cell Signaling), anti-phospho-JNK (1:1000, #9251, Cell Signaling), anti-JNK (1:1000, #9252, Cell Signaling), anti-phospho-Erk1/2 (1:1000, #4370, Cell Signaling), anti-Erk1/2 (1:1000, #9102, Cell Signaling), or anti-β-actin (1:2000, #3700, Cell Signaling) antibody.

Techniques: Western Blot, Phospho-proteomics, Control